FastQCFastQC Report
Mon 6 Feb 2017
SRR2077263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4603850
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.036.484331
GTATCAA99550.034.3664171
TATCAAC136650.024.7446542
ATCAACG135950.024.724593
TCAACGC136950.024.5437834
CAACGCA140600.023.9480635
AACGCAG145450.023.1447356
ACGCAGA170400.019.734337
CGCAGAG174700.019.3280668
GAGTACT119500.019.04010812
AGAGTAC169050.018.9941411
GTACATG115150.018.2950081
GTGGTAT28000.018.160951
AGTACTT127100.018.08427613
CAGAGTA183750.017.73766910
TACATGG116000.017.7043762
GTACTTT129450.017.615414
CGCGTAA1602.934096E-517.26322610
GCAGAGT192650.017.168959
GTATAGG10550.017.0502261
Produced by FastQC (version 0.11.5)