FastQCFastQC Report
Mon 6 Feb 2017
SRR2077249_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077249_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905402
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55000.028.7033081
ATCAACG60350.025.9735953
GGTATCA29750.025.7214321
TCAACGC60300.025.705874
CAACGCA63200.024.689255
AACGCAG66250.023.760136
TATCAAC68300.023.014772
ACGCAGA76400.020.3783847
CGCAGAG77350.019.6820558
AGAGTAC72350.019.5387611
GTACATG75450.017.451521
GAGTACT46900.017.20240212
TACATGG72050.017.0325072
CAGAGTA84000.016.98569910
GTGTAGC23100.016.264611
ACATGGG76200.016.2211463
GCAGAGT90600.015.8885139
GTATAGC14550.015.8723411
AGTACTT53700.015.539309513
GTACTTT52200.015.39172114
Produced by FastQC (version 0.11.5)