FastQCFastQC Report
Mon 6 Feb 2017
SRR2077249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905402
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44950.037.500011
GTATCAA93700.036.0159151
ATCAACG120350.027.690893
TCAACGC120250.027.370714
TATCAAC123900.026.9252452
CAACGCA123300.026.609645
AACGCAG127050.025.7159526
ACGCAGA144350.022.2858377
CGCAGAG143600.022.148898
AGAGTAC142650.021.44999511
GAGTACT122850.020.70930712
CAGAGTA153600.020.10093910
AGTACTT128600.019.70314213
GTACTTT131250.019.52338614
GCAGAGT158450.019.3998669
GTACATG71800.017.6793771
TACATGG69100.017.619132
TACTTTT149000.017.30305515
ACTTTTT155750.017.08724216
ACATGGG73950.016.0915223
Produced by FastQC (version 0.11.5)