FastQCFastQC Report
Mon 6 Feb 2017
SRR2077248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3903426
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62450.030.796341
GGTATCA32500.027.9974141
TCAACGC72550.025.9316884
ATCAACG74150.025.5140933
CAACGCA74050.025.4063995
TATCAAC76750.024.7446732
AACGCAG77800.024.1811776
ACGCAGA86650.021.273777
CGCAGAG87800.020.9170288
GTACATG94600.019.6743321
TACATGG91100.019.5226972
AGAGTAC87700.019.42315111
ACATGGG91050.018.503363
GAGTACT49150.018.17103612
CAGAGTA95600.018.1338810
GCAGAGT101500.017.3461749
GTACTTT55500.016.15579814
TATATCG1958.967891E-615.92784166
CATGGGG52800.015.8870794
AGTACTT57200.015.85631213
Produced by FastQC (version 0.11.5)