FastQCFastQC Report
Mon 6 Feb 2017
SRR2077248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3903426
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.037.0605281
GTATCAA100400.035.2702561
TCAACGC126950.026.6960454
ATCAACG128500.026.6685643
TATCAAC131400.026.2898032
CAACGCA130450.025.8475765
AACGCAG134350.024.9179926
ACGCAGA153050.021.5632297
CGCAGAG156300.020.975688
AGAGTAC149550.020.86051411
GAGTACT122000.020.63768612
CAGAGTA161550.019.50410810
GCAGAGT167350.019.2805889
AGTACTT129950.019.18984413
GTACATG83700.019.0362991
GTACTTT136250.018.4863614
TACATGG82100.018.4398982
ACATGGG84500.016.8165423
TACTTTT156400.016.42138915
ACTTTTT166750.015.81632616
Produced by FastQC (version 0.11.5)