FastQCFastQC Report
Mon 6 Feb 2017
SRR2077247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4649520
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65050.032.6348271
TCAACGC77750.025.837974
ATCAACG79800.025.4786453
CAACGCA80950.024.8586855
GGTATCA34050.024.5944481
TATCAAC85350.024.0652662
AACGCAG84100.023.80196
GTACATG107400.020.825161
ACGCAGA96250.020.6182337
CGCAGAG96000.020.1699148
TACATGG110150.019.2414842
CGCGTAA900.005727956519.17189265
AGAGTAC92050.019.101711
GAGTACT49500.018.4576212
ACATGGG113600.017.8978523
CAGAGTA103750.017.53798510
TCGATCG1653.8469643E-516.72660441
GCAGAGT111650.016.6642069
CATGGGG76950.016.3726924
AGTACTT57450.015.90514513
Produced by FastQC (version 0.11.5)