FastQCFastQC Report
Mon 6 Feb 2017
SRR2077247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4649520
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100500.037.7893681
GGTATCA45350.035.6522671
ATCAACG134500.027.667723
TCAACGC134600.027.36584
TATCAAC136950.027.1228182
CAACGCA138300.026.6585395
AACGCAG142100.025.4373786
ACGCAGA160100.022.2799687
CGCAGAG161750.021.8330848
AGAGTAC157700.021.03893511
GAGTACT119700.020.45097412
CAGAGTA169050.020.21728110
AGTACTT125550.019.68975413
GCAGAGT175850.019.6701269
GTACTTT131650.018.88880514
GTACATG98800.018.2448771
GCGCGTA950.00803188418.0810724
TACATGG99150.017.3093552
TACTTTT150350.016.66436215
ACATGGG104050.016.1634923
Produced by FastQC (version 0.11.5)