FastQCFastQC Report
Mon 6 Feb 2017
SRR2077246_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077246_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160495
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61200.030.8041931
ATCAACG70700.026.0307883
TCAACGC71200.025.9421624
GGTATCA33250.025.395011
CAACGCA73600.025.142835
TATCAAC75350.024.5632532
AACGCAG76700.024.1237186
ACGCAGA85800.021.042367
CGCAGAG88000.020.2828888
GTACATG80300.020.215261
AGAGTAC83850.019.16218411
GAGTACT49750.018.0192712
TACATGG85500.017.8579862
CAGAGTA95050.017.43977210
GTATTAC8050.017.1252961
ACATGGG85850.017.053173
CGATCGT2451.5936166E-816.8948842
GCAGAGT101050.016.5370169
GTACTTT57300.015.94817114
TCGATCG2856.008122E-915.73416441
Produced by FastQC (version 0.11.5)