FastQCFastQC Report
Mon 6 Feb 2017
SRR2077246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160495
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89950.032.987831
GGTATCA46400.031.9005471
ATCAACG115500.024.9704323
TCAACGC114550.024.8169824
TATCAAC118550.024.5605492
CAACGCA117150.024.0605555
AACGCAG121100.023.0768176
ACGCAGA136600.020.4134437
CGCAGAG137400.020.0046398
AGAGTAC132150.019.6771711
GTACATG82150.019.3589611
GAGTACT102350.019.32359912
CAGAGTA147250.018.10381310
TACATGG84500.018.0433122
AGTACTT112650.017.80103913
GCAGAGT151900.017.6865469
GTACTTT114950.017.15244514
ACATGGG86350.016.8593963
TACGGCG1300.00256937615.87985318
CGTTACG1350.003325986715.2766424
Produced by FastQC (version 0.11.5)