FastQCFastQC Report
Mon 6 Feb 2017
SRR2077245_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077245_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613981
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41550.029.109361
GGTATCA24400.027.256121
TCAACGC46950.024.841954
ATCAACG47650.024.8397853
CAACGCA48150.024.0071745
AACGCAG50850.022.6614536
TATCAAC52550.022.36252
GTACATG65200.019.3432771
ACGCAGA60200.018.913067
CGCAGAG60700.018.759618
TACATGG63550.018.5459562
ACATGGG65200.017.2009473
AGAGTAC60650.016.94036111
CAGAGTA67500.015.62270710
GTATAGC13950.015.0678821
GAGTACT39150.014.97133112
GCAGAGT70300.014.8735959
TAGCCCT23450.014.7150494
CATGGGG45600.014.2264834
GTATATA22200.014.1248871
Produced by FastQC (version 0.11.5)