FastQCFastQC Report
Mon 6 Feb 2017
SRR2077245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613981
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.037.8409461
GTATCAA65450.034.4079061
ATCAACG87100.025.3375053
TATCAAC89300.025.0602762
TCAACGC86400.025.0247574
CAACGCA88250.024.420785
AACGCAG92350.023.224756
ACGCAGA105500.020.0491317
CGCAGAG108800.019.2939158
AGAGTAC107700.018.04961611
GAGTACT83000.017.86629112
CAGAGTA115000.017.17407410
GCAGAGT117900.016.9559299
GTACATG64750.016.6985991
AGTACTT91150.016.38205113
GTGGTAT19700.015.9061591
GTACTTT93500.015.90287914
TACATGG63850.015.6911962
TAACCCG2201.6590348E-615.6486625
ACATGGG64700.014.793283
Produced by FastQC (version 0.11.5)