FastQCFastQC Report
Mon 6 Feb 2017
SRR2077244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4726417
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67500.033.6933061
GGTATCA36900.031.2839761
TCAACGC84600.026.0717684
ATCAACG84950.026.0464463
CAACGCA87200.025.4530055
TATCAAC90650.024.9771232
AACGCAG89500.024.3728546
ACGCAGA105400.020.4018237
CGCAGAG107800.019.7885658
AGAGTAC106100.018.39301511
GTACATG110050.018.1610681
TACATGG111300.017.4014742
CAGAGTA117200.016.99575610
GAGTACT59400.016.94988412
GCAGAGT122200.016.3831259
ACATGGG112200.016.3671323
GTGTAGC21700.015.8797531
AGTACTT68100.015.2917713
GTACTTT66650.014.95234814
GTATAGC13000.014.0486951
Produced by FastQC (version 0.11.5)