FastQCFastQC Report
Mon 6 Feb 2017
SRR2077244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4726417
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.10068938056036951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.040.4025341
GTATCAA115250.036.92051
ATCAACG156700.026.5138233
TATCAAC159800.026.3223152
TCAACGC157200.026.254464
CAACGCA159250.025.7860895
AACGCAG163300.025.1255076
ACGCAGA188950.021.5885837
CGCAGAG193450.020.8340388
AGAGTAC187750.020.58366811
GAGTACT149350.020.18995512
CAGAGTA196700.019.64875810
AGTACTT155500.019.43525713
GCAGAGT208250.018.823789
GTACTTT159800.018.74809614
GTACATG109050.018.6108041
TACATGG107100.018.4330332
ACATGGG111100.016.9025783
GTGGTAT27300.016.7582171
TACTTTT181350.016.67804715
Produced by FastQC (version 0.11.5)