FastQCFastQC Report
Mon 6 Feb 2017
SRR2077243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666209
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59400.032.3176651
GGTATCA30400.032.083671
ATCAACG68900.027.2077923
TCAACGC69600.026.9796964
CAACGCA71550.026.0993885
TATCAAC74450.025.3263782
AACGCAG76450.024.4252596
ACGCAGA87400.021.2069177
CGCAGAG89300.020.6019888
GTACATG91550.019.4250681
AGAGTAC90450.018.75567811
TACATGG92250.018.2722872
CAGAGTA99100.017.45752310
ACATGGG94000.017.224943
GAGTACT58550.016.96080812
GCAGAGT103150.016.8993329
CGTCGTA1756.421964E-515.75449610
GTGTAGC26450.015.636041
AGTACTT66850.015.37259113
GTATAGC16500.015.2479021
Produced by FastQC (version 0.11.5)