FastQCFastQC Report
Mon 6 Feb 2017
SRR2077243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666209
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.041.4758381
GTATCAA97500.035.4167021
ATCAACG125650.026.9003683
TCAACGC125700.026.6434754
TATCAAC129250.026.2314852
CAACGCA127800.026.1249565
AACGCAG133700.024.920666
ACGCAGA154500.021.4635017
CGCAGAG155700.020.9796148
AGAGTAC156650.019.77344911
GAGTACT128250.018.97879212
CAGAGTA165700.018.94326810
GCAGAGT170850.018.4736619
AGTACTT136450.017.8632613
GTACATG85950.017.5269851
GTACTTT140200.017.19648614
TACATGG87750.016.653992
GTGTAGC25250.015.9368871
ACATGGG89800.015.2396133
TACTTTT162300.015.09864315
Produced by FastQC (version 0.11.5)