FastQCFastQC Report
Mon 6 Feb 2017
SRR2077242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904752
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63950.032.2860071
TCAACGC75800.026.1423784
ATCAACG76350.025.8666133
CAACGCA78850.025.1749465
TATCAAC80500.025.1367592
GGTATCA33600.025.0310251
AACGCAG82150.024.2022616
AGAGTAC86300.021.09796311
ACGCAGA92750.020.8411057
GAGTACT46850.020.24139812
CGCAGAG93850.020.2318868
GTACATG91800.018.9616781
CAGAGTA100100.018.48943910
CGACGTA1150.001106972818.00943256
TACATGG91850.017.9772572
GTACTTT53400.017.7622514
AGTACTT54300.017.7207913
GCAGAGT103450.017.5868519
ACATGGG94350.016.723493
TCGATCG2503.6872916E-715.17567841
Produced by FastQC (version 0.11.5)