FastQCFastQC Report
Mon 6 Feb 2017
SRR2077242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904752
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40280.10315635922588681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48550.038.7795221
GTATCAA102200.034.5878751
ATCAACG133500.025.9066473
TCAACGC132850.025.8520724
TATCAAC136300.025.4754412
CAACGCA138000.024.786925
AACGCAG140000.024.187026
ACGCAGA161000.020.716757
CGCAGAG164300.020.0186798
AGAGTAC158950.019.56518711
GAGTACT123350.019.15991812
CAGAGTA172600.018.37805710
GCAGAGT174100.018.2985299
GTACATG89900.018.2626191
AGTACTT131900.018.17875313
GTACTTT134300.017.78319714
TACATGG92000.017.2066542
ACCGTAT4400.016.420328
GTACCGT3651.8189894E-1216.0277046
ACATGGG92050.015.7392743
Produced by FastQC (version 0.11.5)