FastQCFastQC Report
Mon 6 Feb 2017
SRR2077241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5157694
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68400.031.594491
GGTATCA36000.030.7328241
TCAACGC77600.026.6457144
ATCAACG78650.026.2933083
CAACGCA81750.025.123915
TATCAAC86050.024.5141772
AACGCAG84800.024.1783986
CGACCGA800.002897746221.56639966
ACGCAGA96300.020.7894447
CGCAGAG96450.020.6859368
AGAGTAC94800.019.53258911
GTACATG101150.019.4226681
TACATGG101550.018.6001912
ACATGGG100500.017.9660573
GAGTACT60150.017.5996912
CAGAGTA109350.017.39647310
GCAGAGT112900.016.8473879
GTATAGC18350.016.7168271
CATGGGG68800.015.8548044
TAGCCCT33500.015.66249754
Produced by FastQC (version 0.11.5)