FastQCFastQC Report
Mon 6 Feb 2017
SRR2077241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5157694
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51650.037.5532151
GTATCAA115700.036.708951
ATCAACG149500.027.921313
TCAACGC149050.027.8441244
TATCAAC153000.027.4401652
CAACGCA153300.027.0719285
AACGCAG158100.026.097776
ACGCAGA181150.022.5877827
CGCAGAG183300.022.1495868
AGAGTAC179800.021.39302611
GAGTACT144300.020.60542712
CAGAGTA194450.019.94113310
GCAGAGT198100.019.7996859
AGTACTT155150.019.38580713
GTACATG105700.019.326351
GTACTTT162000.018.46914514
TACATGG106100.018.342712
TATCTCG4400.018.00481236
GTATAGC19250.017.8651891
ACATGGG106200.017.2892043
Produced by FastQC (version 0.11.5)