FastQCFastQC Report
Mon 6 Feb 2017
SRR2077240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4367809
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54100.031.4068151
GGTATCA27750.029.6830921
TCAACGC60750.027.278874
ATCAACG61150.027.1593863
CAACGCA63600.025.7304675
TATCAAC66200.025.0936182
AACGCAG66750.024.4110956
TATCGCG750.001985722523.0162835
TATACCG3000.021.8654695
ACGCAGA73300.021.7121817
CGCAGAG75250.021.014568
AGAGTAC80100.018.16533311
GTACATG80650.018.1190621
TACATGG78900.017.2978632
CGATCGA1200.00148761717.23273510
CAGAGTA88100.016.74372310
GTGTAGC27350.016.5077611
ACATGGG79150.016.489683
GCAGAGT91400.016.3195749
GTATAGC15950.015.7738041
Produced by FastQC (version 0.11.5)