FastQCFastQC Report
Mon 6 Feb 2017
SRR2077240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4367809
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.039.528131
GTATCAA86400.036.3005331
ATCAACG114750.027.1453083
TCAACGC114750.026.9653384
TATCAAC117150.026.6776392
CAACGCA117000.026.4467745
AACGCAG122650.025.0320346
ACGCAGA138200.021.7783647
CGCAGAG138850.021.5653888
AGAGTAC143000.019.99995411
GAGTACT115250.019.61952212
CAGAGTA151900.018.91919510
AGTACTT122350.018.76229113
GCAGAGT155650.018.596279
GTACTTT125000.018.20454614
GTACATG83800.016.9262811
TACTTTT145150.015.95026815
ACTTTTT148250.015.91985716
TACATGG78750.015.9094312
ACATGGG79550.015.2733163
Produced by FastQC (version 0.11.5)