FastQCFastQC Report
Mon 6 Feb 2017
SRR2077239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200747
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62350.032.940591
GGTATCA33250.031.7140241
TCAACGC75750.026.7030934
ATCAACG76450.026.549843
CAACGCA78100.025.8557175
TATCAAC80450.025.3189162
AACGCAG81100.024.8977936
ACGCAGA94500.021.1118877
CGCAGAG96450.020.4713048
AGAGTAC93000.019.093211
GTACATG100300.018.6217271
CAGAGTA102100.018.15993710
GAGTACT54050.018.05282812
TACATGG98300.017.7060432
GCAGAGT109050.017.3142249
TATACTG15450.016.9799675
ACATGGG105400.016.4408863
AGTACTT64000.015.89417413
GTATAGC14300.015.6638631
GTACTTT62450.015.62682114
Produced by FastQC (version 0.11.5)