FastQCFastQC Report
Mon 6 Feb 2017
SRR2077239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200747
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.039.695061
GTATCAA101500.036.9836651
TATCAAC132450.027.8953022
TCAACGC130700.027.7423844
ATCAACG132900.027.5678833
CAACGCA134500.026.9067865
AACGCAG138550.025.9464656
ACGCAGA156900.022.6505077
CGCAGAG158900.022.2275058
AGAGTAC157100.021.2972711
GAGTACT119800.020.66631112
CAGAGTA167900.020.11262510
GCAGAGT173950.019.5924249
AGTACTT128400.019.57647113
GTACTTT129800.019.37340214
GTACATG96600.017.8448051
TACTTTT146300.017.22232215
TACATGG96000.016.9499592
ACTTTTT156950.016.7348216
TATACCG2804.9258233E-915.9717955
Produced by FastQC (version 0.11.5)