FastQCFastQC Report
Mon 6 Feb 2017
SRR2077238_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077238_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686937
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.032.1960751
GTATCAA59450.030.7829861
ATCAACG71850.024.935093
TCAACGC71400.024.8471284
TATCAAC74300.024.4473112
CAACGCA73200.024.3297775
AACGCAG76300.023.3403266
GTACATG101550.020.5665171
ACGCAGA90150.019.4101947
TACATGG104000.019.3879492
CGCAGAG91000.018.8501348
ATACCGA1903.488949E-718.1646986
ACATGGG109050.017.7902073
AGAGTAC87900.017.72749311
CAGAGTA96300.016.53309810
GAGTACT42450.016.24261712
GCAGAGT101500.015.9826579
CGTATAC1300.00251340615.9322913
CCGTCGA1300.002515459415.9303429
CATGGGG63500.014.730144
Produced by FastQC (version 0.11.5)