FastQCFastQC Report
Mon 6 Feb 2017
SRR2077238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686937
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45450.038.9720041
GTATCAA95150.036.1468621
TATCAAC126200.026.9506452
ATCAACG126100.026.89023
TCAACGC126000.026.5294284
CAACGCA127600.026.0620175
AACGCAG131850.024.9611176
ACGCAGA150150.021.6936197
CGCAGAG154100.020.7918038
GTACATG97350.020.491391
AGAGTAC149900.020.06304711
GAGTACT106400.019.54358312
TACATGG100950.019.5200332
CAGAGTA160150.018.90927110
AGTACTT113900.018.5282813
GCAGAGT166450.018.4217329
ACCGTAT3400.018.2000458
GTACTTT116900.017.94151314
GTGGTAT20150.017.9222971
ACATGGG107300.017.2110273
Produced by FastQC (version 0.11.5)