FastQCFastQC Report
Mon 6 Feb 2017
SRR2077237_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077237_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475612
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43400.030.972751
GGTATCA22400.030.4664671
ATCAACG49000.026.0019173
TCAACGC48600.025.8569624
TATCAAC52750.024.6990642
CAACGCA51000.024.504785
AACGCAG52200.023.6087276
GTACATG72500.019.9671551
ACGCAGA62200.019.4803987
TACATGG71750.018.7350082
CGCAGAG65150.018.4934188
GAGTACT34750.017.9575712
ACATGGG70750.017.910793
GTATAGC12050.017.4480821
AGAGTAC63950.017.19784211
CAGAGTA72000.015.65268710
CATGGGG47600.015.4484564
AGTACTT41350.015.00987613
GCAGAGT76250.014.9423149
GTACTTT41300.014.77821914
Produced by FastQC (version 0.11.5)