FastQCFastQC Report
Mon 6 Feb 2017
SRR2077237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475612
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35450.037.11
GTATCAA74650.033.4837071
ATCAACG92850.025.9907653
TCAACGC92550.025.8518354
TATCAAC95550.025.6169912
CAACGCA93750.025.4474895
AACGCAG97650.024.2196276
ACGCAGA110800.020.8017147
CGCAGAG111900.020.3952438
GAGTACT83500.019.90901612
AGAGTAC110400.019.70321711
AGTACTT88800.018.64323413
CAGAGTA120600.018.06661610
GTACTTT91650.017.84356514
GTACATG69050.017.7505991
GCAGAGT125000.017.6792649
TACATGG67650.017.4548262
GCGAATC4250.017.07083933
GGCGAAT4600.015.86576832
TATACCG1756.480813E-515.7374695
Produced by FastQC (version 0.11.5)