FastQCFastQC Report
Mon 6 Feb 2017
SRR2077236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4547627
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74800.033.9570961
GGTATCA36750.032.353881
TCAACGC93050.026.8970074
ATCAACG93700.026.7130723
CAACGCA94950.026.5399845
TATCAAC98400.025.6044582
AACGCAG99750.025.156836
ACGCAGA115900.021.2639737
CGCAGAG117900.020.817278
AGAGTAC115500.019.40339511
GTACATG121950.019.0477091
TATACCG2002.8667273E-818.9860845
TACATGG124200.018.1766152
CAGAGTA127950.017.85722410
GAGTACT58950.017.66339712
GCAGAGT133450.017.3582299
ACATGGG126850.017.3370173
AGTACTT66650.016.19376813
GTCGTAC1959.083789E-615.9064231
TGCGCGA1554.5467855E-415.572062552
Produced by FastQC (version 0.11.5)