FastQCFastQC Report
Mon 6 Feb 2017
SRR2077236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4547627
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50380.11078305234796082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55900.038.9605181
GTATCAA121750.036.143851
TATCAAC161900.026.8365762
ATCAACG161750.026.818933
TCAACGC160950.026.6316264
CAACGCA163200.026.179575
AACGCAG167950.025.1524016
ACGCAGA191700.021.8755747
CGCAGAG194050.021.2531648
AGAGTAC187400.020.73561911
GAGTACT144650.020.44504712
AGTACTT152600.019.71772813
GTACATG113750.019.4185331
CAGAGTA201450.019.41041210
GCAGAGT205250.019.2205099
GTACTTT158800.018.8493714
TACATGG114300.018.6601922
ACATGGG117500.017.2736763
ACTTTTT186000.016.56490116
TACTTTT180150.016.52580815
Produced by FastQC (version 0.11.5)