FastQCFastQC Report
Mon 6 Feb 2017
SRR2077235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4423783
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37000.032.6962171
GTATCAA69400.031.535981
TCAACGC83600.025.5163464
ATCAACG84100.025.2027573
CAACGCA86500.024.5813565
TATCAAC88700.024.2095092
AACGCAG89500.023.4472666
CGCGTAA800.00291827721.54008910
ACGCAGA102550.020.0598287
GTACATG103600.019.7947181
TACATGG99700.019.5678372
CGCAGAG104300.019.4596168
AGAGTAC99700.018.71073211
ACATGGG106650.018.06133
CAGAGTA110150.017.3337610
GAGTACT59000.017.066112
GCAGAGT116550.016.7031449
GTACTTT64800.015.912518514
CATGGGG72600.015.5945744
AGTACTT66200.015.5233813
Produced by FastQC (version 0.11.5)