FastQCFastQC Report
Mon 6 Feb 2017
SRR2077235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4423783
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55500.039.724881
GTATCAA114950.035.218031
TATCAAC149650.026.771522
ATCAACG147950.026.567773
TCAACGC148250.026.3052374
CAACGCA151850.025.5451435
AACGCAG156900.024.5256846
ACGCAGA181050.020.971247
CGCAGAG182900.020.5819578
AGAGTAC178500.019.98923911
GAGTACT140000.019.88813812
GTACATG105650.019.6311341
TACATGG101350.019.544412
AGTACTT147150.019.13201713
CAGAGTA191300.018.8684410
GTACTTT150000.018.77594214
GCAGAGT196850.018.4942639
ACATGGG104400.017.985233
TACTTTT166100.017.1525515
GTGGTAT25050.016.888651
Produced by FastQC (version 0.11.5)