FastQCFastQC Report
Mon 6 Feb 2017
SRR2077234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612695
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.030.2858091
GTATCAA47000.030.1391
ATCAACG54250.024.9497573
TCAACGC54050.024.848364
CAACGCA55800.023.7593655
TATCAAC58600.023.0563952
AACGCAG58700.022.6415526
ACGCAGA66300.019.5775287
CGCAGAG68000.018.7855788
AGAGTAC66200.018.02244411
GTATAGC11800.017.2327611
GAGTACT44200.017.00660912
TAGCCCT24150.016.7267884
GTACATG62450.016.6670741
TACATGG61100.016.3591122
CAGAGTA75500.016.25106810
GCAGAGT80300.015.0920229
AGTACTT49800.014.956984513
ACATGGG62250.014.9208283
GTGTAGC19000.014.3304011
Produced by FastQC (version 0.11.5)