FastQCFastQC Report
Mon 6 Feb 2017
SRR2077234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612695
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.039.500541
GTATCAA76500.034.4982641
TCAACGC96300.026.5410124
ATCAACG97700.026.3719463
TATCAAC100350.025.8472842
CAACGCA98400.025.8347515
AACGCAG102650.024.5305396
ACGCAGA115250.021.594337
CGCAGAG116550.021.1277568
AGAGTAC116850.019.74632511
GAGTACT100350.018.64120712
GTACATG60400.018.5144021
TACATGG58900.018.4557932
CAGAGTA125600.018.39959310
GCAGAGT129400.018.1518559
AGTACTT106400.017.6135713
GTACTTT109600.017.32660914
CGGCGCG1200.001505993317.20105414
ACATGGG61250.016.4557023
TATACCG2101.0297554E-616.380825
Produced by FastQC (version 0.11.5)