FastQCFastQC Report
Mon 6 Feb 2017
SRR2077233_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077233_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5260718
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76550.029.670361
TCAACGC85750.025.4015464
ATCAACG86400.025.212613
CAACGCA88500.024.84655
GGTATCA43350.023.692441
AACGCAG93400.023.3555246
TATCAAC95050.023.0995222
ACGCAGA106050.020.3092237
CGCAGAG106200.020.086498
GTACATG103850.019.4811331
AGAGTAC102100.018.8790211
TACATGG103950.018.5354422
GAGTACT59600.017.70388412
CAGAGTA118050.016.99132310
ACATGGG109750.016.797283
GCAGAGT126100.015.9600769
AACGTCG1554.529779E-415.57981722
GTACTTT68300.015.29848514
GGACCGT4300.015.2512196
AGTACTT73100.014.90720413
Produced by FastQC (version 0.11.5)