FastQCFastQC Report
Mon 6 Feb 2017
SRR2077233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5260718
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.036.0644341
GTATCAA118650.034.080671
TCAACGC149700.025.9722844
ATCAACG150850.025.9112663
CAACGCA153050.025.3139515
TATCAAC156700.025.2076742
AACGCAG158650.024.2254076
ACGCAGA179900.021.1022017
CGCAGAG182500.020.6447098
AGAGTAC171850.020.30033511
GAGTACT135100.019.56400312
CAGAGTA189700.018.78947610
AGTACTT147700.018.40698613
GCAGAGT198050.018.1201559
GTACATG102750.017.8701421
GTACTTT151550.017.6292414
TACATGG101850.016.8768232
CGTATAC1855.6422195E-616.7241573
GTATAGC16450.016.5131441
ACATGGG104400.016.233793
Produced by FastQC (version 0.11.5)