FastQCFastQC Report
Mon 6 Feb 2017
SRR2077232_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077232_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589393
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61350.031.3503551
GGTATCA33700.028.1271421
TCAACGC69400.026.507224
ATCAACG70750.026.3942893
CAACGCA71300.025.6556345
TATCAAC74850.025.235482
AACGCAG76800.023.8170176
ACGCAGA87950.020.3273547
CGCAGAG88850.019.850198
GTACATG91150.018.7185131
AGAGTAC88300.018.35239211
TACATGG88600.018.2846622
GAGTACT54000.017.24028612
CAGAGTA99950.016.89581910
ACATGGG91800.016.694733
GTATAGC16050.016.5363061
GCAGAGT103000.016.4198639
GTGTAGC23800.015.9308351
TAGCCCT27550.015.7850284
CATGGGG61200.015.1140134
Produced by FastQC (version 0.11.5)