FastQCFastQC Report
Mon 6 Feb 2017
SRR2077232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589393
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94950.036.025271
GGTATCA45600.035.318061
ATCAACG122950.027.1448823
TCAACGC123050.026.9830174
TATCAAC126500.026.628192
CAACGCA126850.025.929735
AACGCAG130200.025.0775896
ACGCAGA149350.021.5247737
CGCAGAG152750.020.6763118
AGAGTAC150200.019.87902611
GAGTACT117400.019.54353112
GTACATG92600.018.6927341
CAGAGTA162200.018.66403410
TACATGG91650.018.2453982
GCAGAGT167250.018.1420219
AGTACTT127350.018.01637613
GTACTTT129350.017.8220614
CGATCGT2208.098323E-817.33884242
TATACCG2601.8389983E-917.2028565
ACATGGG94750.017.0672453
Produced by FastQC (version 0.11.5)