FastQCFastQC Report
Mon 6 Feb 2017
SRR2077231_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077231_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4596826
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54250.029.408311
GGTATCA30900.028.9937041
TCAACGC65400.023.385574
ATCAACG66350.023.2608993
TATCAAC69150.022.2755182
CAACGCA69000.022.164975
AACGCAG71600.021.262276
TACATGG88800.019.2089982
GTACATG93850.019.0922831
ACATGGG92150.018.3594913
ACGCAGA82900.017.9478137
CGCAGAG84150.017.517888
GTATACG3501.8189894E-1216.7366961
GTGTAGC27450.016.193331
AGAGTAC83950.015.97601311
GAGTACT49550.015.37808912
GTATAGG13000.015.3735261
CATGGGG59150.014.9419474
CAGAGTA92250.014.79087810
GTATATA28300.014.4893661
Produced by FastQC (version 0.11.5)