FastQCFastQC Report
Mon 6 Feb 2017
SRR2077231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4596826
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93700.035.341581
GGTATCA45500.034.7652741
ATCAACG117500.027.653533
TATCAAC118250.027.5653742
TCAACGC117650.027.4136964
CAACGCA120850.026.573425
AACGCAG125250.025.5026446
ACGCAGA143750.021.914687
CGCAGAG144800.021.5295318
GAGTACT105700.020.83958612
AGAGTAC143500.020.35486811
CAGAGTA151500.019.4854410
AGTACTT116200.019.19307513
GCAGAGT160200.018.7502449
GTACTTT118800.018.6377314
TACATGG90450.017.7527242
GTACATG96300.017.7114721
ACATGGG94150.016.4707323
TACTTTT138050.016.1272215
ACTTTTT146800.015.73142816
Produced by FastQC (version 0.11.5)