FastQCFastQC Report
Mon 6 Feb 2017
SRR2077230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233572
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46650.031.0281071
GGTATCA25000.026.6057131
ATCAACG53250.026.1229063
TCAACGC53500.025.8705734
CAACGCA55800.024.9900885
TATCAAC59900.023.6473732
AACGCAG59500.023.375586
ACGCAGA69400.019.8918347
CGCAGAG69900.019.6521978
GTACATG78100.019.3277231
TACATGG76750.018.1414032
ACATGGG76500.017.5519053
AGAGTAC72950.016.96846411
GTATAGC15100.016.2046261
GCAGAGT82450.015.699549
CAGAGTA80000.015.63793510
GAGTACT44850.015.37609612
TAGCCCT27600.014.7566714
CATGGGG49850.014.3324584
CGTGCGC6500.014.31571910
Produced by FastQC (version 0.11.5)