FastQCFastQC Report
Mon 6 Feb 2017
SRR2077230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233572
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.037.7376861
GTATCAA81200.037.02591
ATCAACG98850.029.5726183
TCAACGC99100.029.3244974
TATCAAC103350.028.5515372
CAACGCA101850.028.3976555
AACGCAG105800.027.1424066
ACGCAGA126050.022.6297577
CGCAGAG126800.022.2674988
AGAGTAC126000.021.04066511
GAGTACT98350.020.73270412
CAGAGTA134600.020.00381910
GCAGAGT140750.019.3261269
AGTACTT108800.018.77253513
GTACTTT109850.018.569714
GTACATG75550.018.1216961
TACATGG75950.017.0260052
ACATGGG78200.016.0081883
TACTTTT129200.015.77605515
ACTTTTT136500.015.56371916
Produced by FastQC (version 0.11.5)