FastQCFastQC Report
Mon 6 Feb 2017
SRR2077229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799571
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30000.030.0948891
TCAACGC33350.027.0119934
CAACGCA33850.026.8169295
ATCAACG34200.026.240193
AACGCAG35650.025.3651946
TATCAAC38000.023.0332432
GGTATCA16700.021.4599951
ACGCAGA43100.020.8205667
CGCAGAG43850.019.9925568
GTACATG45350.019.6804581
TACATGG44750.018.7119142
AGAGTAC42700.017.68229511
TACACCG2005.968086E-717.2576645
GTATAGC10400.016.8985561
ACATGGG46500.016.849723
CAGAGTA49950.015.79753910
GCAGAGT50250.015.66068659
GAGTACT28600.015.43084212
ACCGTCG1350.003235396715.339878
CGTGCGC4051.8189894E-1215.31464410
Produced by FastQC (version 0.11.5)