FastQCFastQC Report
Mon 6 Feb 2017
SRR2077229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799571
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50150.036.5338751
GGTATCA23300.034.2915461
ATCAACG63400.027.9706843
TCAACGC62850.027.6675824
TATCAAC66000.027.3383622
CAACGCA65050.026.7847985
AACGCAG67850.025.4764546
ACGCAGA79100.021.6615077
CGCAGAG80400.020.8967638
GAGTACT63400.020.6326612
AGAGTAC80200.020.17328511
CGTATAC1551.0242184E-619.9938263
CAGAGTA84600.019.36995110
AGTACTT69800.019.03674713
GTACTTT71000.018.67118814
GCAGAGT89950.018.3709669
ATAACGC1150.001125168417.9654673
GTACATG49950.017.9263921
TACATGG48200.017.6455442
GTATAGG6950.016.35251
Produced by FastQC (version 0.11.5)