FastQCFastQC Report
Mon 6 Feb 2017
SRR2077228_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077228_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4690844
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69900.027.9091531
GGTATCA40950.026.00831
TCAACGC82400.022.4085524
ATCAACG84350.022.0574823
CAACGCA85750.021.5333465
TATCAAC89000.021.1072882
AACGCAG88750.020.64776
GTACATG97500.018.7714271
TACATGG96750.017.8133262
ACGCAGA101700.017.7809857
CGCAGAG102850.017.4494468
AGAGTAC95550.016.99597211
GAGTACT56350.016.58195712
ACATGGG100150.016.0270733
CAGAGTA109750.015.3542410
GTATAGG12050.014.873861
GTACTTT63400.014.8493714
AGTACTT65500.014.84696913
GCAGAGT118050.014.6761139
CATGGGG68450.013.6641644
Produced by FastQC (version 0.11.5)