FastQCFastQC Report
Mon 6 Feb 2017
SRR2077228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4690844
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56250.035.100851
GTATCAA110350.033.1975141
ATCAACG139600.025.570993
TCAACGC139200.025.471534
TATCAAC144150.025.0268212
CAACGCA142750.024.7414575
AACGCAG146600.023.9744076
CGGTACG750.00203120722.9268474
ACGCAGA166100.020.7513927
CGCAGAG167500.020.3264058
AGAGTAC157450.020.24604611
GAGTACT124400.019.95813612
GTACATG96600.019.8694271
TACATGG95700.019.0462192
CAGAGTA174200.018.63569310
AGTACTT133850.018.52337313
GCAGAGT180250.018.2584119
GTACTTT136900.018.04534514
ACATGGG101150.017.2716333
GTACCGT3950.016.5419796
Produced by FastQC (version 0.11.5)