FastQCFastQC Report
Mon 6 Feb 2017
SRR2077227_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077227_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3720252
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62450.032.3388861
GGTATCA35550.028.3076081
TCAACGC75150.025.8551834
ATCAACG76700.025.4687023
TATCAAC78150.025.0066132
CAACGCA79000.024.8132465
AACGCAG81400.023.7418336
GTACATG85400.019.975941
ACGCAGA95100.019.8133037
TACATGG85850.019.431482
CGCAGAG97100.018.944458
AGAGTAC87400.018.9380511
GAGTACT53100.018.24808312
CAGAGTA99000.017.4406410
ACATGGG89800.017.3334883
TATACCG2301.4199031E-716.5054445
GCAGAGT107250.016.476039
AGTACTT61100.016.1987413
GTACTTT59850.016.13371714
CGACTAA1756.397621E-515.76133650
Produced by FastQC (version 0.11.5)