FastQCFastQC Report
Mon 6 Feb 2017
SRR2077227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3720252
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45700.037.4764751
GTATCAA90550.034.5999531
TATCAAC116950.026.4928872
ATCAACG117450.026.2919123
TCAACGC116850.026.249994
CAACGCA120100.025.5099835
AACGCAG123900.024.8108526
ACGCAGA143300.021.0496127
CGCAGAG144650.020.4871968
AGAGTAC138700.020.07418611
GAGTACT107950.019.61496212
GTACATG84600.019.1061461
CAGAGTA150750.018.90409310
AGTACTT113200.018.7353713
TACATGG83150.018.6517242
GCAGAGT155750.018.518439
GTACTTT116300.018.27364214
ACATGGG88000.017.076453
TACTTTT129100.016.42239215
ACTTTTT137400.015.93824516
Produced by FastQC (version 0.11.5)