FastQCFastQC Report
Mon 6 Feb 2017
SRR2077226_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077226_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877291
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.032.6295931
GTATCAA28700.031.4654481
TCAACGC34950.024.7901134
ATCAACG35050.024.3287013
CAACGCA36350.024.0252595
TATCAAC36900.023.634342
AACGCAG38900.022.4473446
GCGTAAC850.004143422520.2752231
ACGCAGA44300.019.477897
CGCAGAG44900.019.1417588
GTACATG43550.018.6783471
GAGTACT25650.018.27975512
AGAGTAC43600.018.18647811
TACATGG42650.018.0233352
TACTCCG1602.9383924E-517.2592535
CAGAGTA47250.017.06707610
ACATGGG43900.016.9863173
ATACCGT1250.001936006116.5666716
GCAGAGT50500.016.3351739
AGTACTT29650.016.16337613
Produced by FastQC (version 0.11.5)