FastQCFastQC Report
Mon 6 Feb 2017
SRR2077226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877291
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.040.5425951
GTATCAA47350.037.420081
ATCAACG63250.027.3539873
TCAACGC63800.027.0103534
TATCAAC64700.027.006772
CAACGCA65850.026.1172495
AACGCAG66600.025.4616096
ACGCAGA75550.022.0214657
CGCAGAG76000.021.5894288
AGAGTAC74750.020.75012211
GAGTACT60050.020.50337412
TCGTATG2603.6379788E-1220.06668740
CAGAGTA78200.019.92475910
AGTACTT63650.019.66738913
GCAGAGT82100.019.1473839
GTACTTT65900.019.10688814
GGACCGT1451.2987402E-518.9773336
TATGCCG2951.8189894E-1218.82383743
TACACCG2751.0913936E-1118.7616835
TACATGG39150.018.0108972
Produced by FastQC (version 0.11.5)