FastQCFastQC Report
Mon 6 Feb 2017
SRR2077225_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077225_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4900559
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70550.032.1942331
GGTATCA36150.029.1744611
ATCAACG81550.026.6251013
TCAACGC81500.026.5971764
CAACGCA83950.025.8206965
TATCAAC86950.025.3703632
AACGCAG87800.024.450096
ACGCAGA98550.021.5028677
CGCAGAG101000.020.504548
AGAGTAC100350.018.69494411
GTACATG97600.018.6454891
GTGTAGC26100.018.09131
TACATGG96700.018.000362
GTATAGC18200.017.9904461
GAGTACT61400.017.63615612
CAGAGTA109650.017.47494910
GCAGAGT113450.016.9160659
TAGCCCT33900.016.9021474
ACATGGG99200.016.49423
GTACTTT67850.015.9105814
Produced by FastQC (version 0.11.5)